Fig. 2a . Three of the skin flaps are mobilized in the dorsal aspects of the rats.
Each flap presents 5 cm length and 1 cm in the base. The flaps vertex are placed cranially.
Fig. 2b - The skin flaps after undermined are placed in their normal position. At the vertex "a" Gillis isolated type of stitch with 5-0 mono filament nylon is placed and an isolated stitch in each flap side complete the suture.
Fig. 2c - All the triangular flaps present 16% of necrosis of their distal portion, 24 hours after their mobilization.
Fig. 2d - At the 8th
postoperative day, the flap "A" treated with ACE Pool shows significant necrosis regression, while the flap "B" presents a necrotic scar.
Fig. 2c - Microscopic aspect of the flap C (control) 24 hours after its mobilization, showing dermo epidermic necrosis with acute inflammatory reaction, with the presence of capillary network dilation, high neurrophil concentration around the vessels, necrotic phanerous and neotrophils surrounding the hair axis.
Fig. 2f - Microscopic aspects of the non treated flap (B) after 7 postoperatory days, showing the dissociation of the corneal stratus done by ncutrophils and red cells, epidermic necrotic focus, neutrophils exudate below the dermal level derno epidermic necrosis focus of tissue regeneration and inflammatory reactions.
Fig. 2g - Microscopic aspects of the treated flap (A) after 7 days postoperarive, showing a thick corneal stratum with regenerate epidermis, the dermis with presence of cutaneous annex, ectasia of capillary network with few surrounding neutrophils, fibroblast proliferation and histioid cells inside the fibrin network.
Fig. 3a - A 51 year-old white woman after a routine rhytidectomy,shows a retro-auricular skin necrosis behind the ear 24, hours after the surgery.
Fig. 3b Same patient 14 days after daily injection of face pool. complete skin regeneration is observed without evidence of scarring.
Fig. 4a - A 33 year-old woman, after breast reduction through vertical incision procedure, shows vascular dcficiency ar the areola-nipple complex 24 hours after surgery.
Fig. 4b - Same patient 14 days after daily injection of ACE pool. A complete regeneration of the areola-nipple complex is observed, without suture dehiscence or scar deformity.
Figs. 5a & 5b - A 50 year-old man 24 hours after rhytidccromy, shows a huge hematoma with skin vascular deficiency.
Fig. 5c - 4 days after daily injection of ACE pool.
Fig. 5d - 15 days after daily injection of ACE pool.